Introduction to
BARLEY GERMPLASM CENTER

Research Institute for Bioresources
Okayama University
Japanese homepage



Research Institute for Bioresources, Okayama University
2-20-1, Chuo, Kurashiki, 710-0046, JAPAN
Tel. 086-424-1661 (International +81-86-424-1661)
Fax. 086-434-1249 (International +81-86-434-1249)
e-mail kazsato@rib.okayama-u.ac.jp (curator) 



History

@@@Early in 1940's Dr. Ryuhei Takahashi, Emeritus Professor of Okayama University, started the collection of barley varieties from all over the world. By the support of his successors the collection increased year by year. In 1979 the Ministry of Education, Science, Sports and Culture established the Barley Germplasm Center at the Institute for Agricultural and Biological Sciences (renamed as Research Institute for Bioresources in 1989), Okayama University located at Kurashiki. In 2003 four staff members, ten full-time and part-time assistants, two PhD and one graduate students are involved in the germplasm project. Several cooperative research works are conducted with the scientists in and out of the Institute.
 


Research Institute for Bioresources 


Barley Germplasm Center


Barley

@@@Barley is the fourth major cereal crop in the world. About 8,000 years ago it was domesticated from a wild ancestor in the Fertile Crescent and now it is grown throughout the world excepting the tropics because of its wide adaptability to the salt, drought, low temperature etc. The barley varieties show a large variation in morphological, ecological and agricultural traits such as plant type, ear type, growth habit, maturity, plant height, grain size, disease resistance etc. About 2,000 years ago barley was introduced to Japan from the continent, and it used to be an important food crop. Barley was cooked with rice when the rice production was limited, and sometimes we eat barley in this way still now. At present barley is used mainly as feed of livestocks, malts for beer and whisky, miso, soy-source and Mugicha-tea. Recently the consumers attend to Mochi-mugi or waxy barley because of its special function as a diet.
 


Old Methopotamian cray on which barley images are drawn(exhibition in British Museum)@ 


Barley chromosomes (2n=14)


Non-brittle rachis in cultivated barley (left) and brittle rachis in wild barley(right)


Germplasm

@@@During the long process of evolution, the genetic diversity of organisms has been expanded very much. The genetic diversity is the essential base of the biosphere where the human beings are living, and it is also the principal factor for improving the genetic ability of the organism through the breeding for the human welfare. Therefore we have to maintain and expand the germplasm for the future.
 


Variation of triplets in barley 


Morphological variation of spike 
types from a Tibetan field


Objectives

@@@The objectives of the Center is (1) to collect and preserve the barley germplasm, (2) to evaluate and analyze the genetic feature of the materials, (3) to constract database and seed stocks for worldwide distribution. At the beginning of the Center the number of collection was about 5,000 and it has grown up to 10,000. During the recent five years (1998-2002) the Center introduced ca. 1,600 samples and distributed ca. 7,300 samples.
 

 Sampling in Tibetan landrace field

 UNIX workstation for database 
management


Evaluation and preservation of the materials

@@@At Kurashiki where the winter is so mild, barley is usually sown in the late autumn. After germination many plant characters such as plant type, hairiness of leaf sheath, heading time, ear type, awn length, hull type etc. are carefully checked by experienced staffs. The barley varieties head in April and mature in late May. Then barley spikes are harvested, dried and threshed very carefully to prevent the contamination. After fumigated with chemical insecticide the seeds are stored in storage room at 15Ž (short and middle term) or -30Ž (long term) under dry condition. The preserved materials will be distributed with the request of scientific purpose.
 

@

@

@

@

@

@

Barley genotypes tolerant (left) and susceptible (right) to excessive moisture


Bagging to prevent outcrossing 


Seed storage room


Contents of the germplasm

@@@About 5,200 cultivars originated from various regions of the world, 274 wild barley lines, 421 mutants, 172 linkage testers, 13 trisomics and 377 isogenic lines are registered. In addition more than 2,000 cultivars and thousands of mutagen treated lines are under evaluation.
 

Seed multiplication of wild species

Chlorophyll mutants

Ears of addition lines (1-7) of wheat (W) to which each chromosome of barley (B) is introduced 


Person in Charge

Kazuyoshi Takeda (Professor, Head) takeda@rib.okayama-u.ac.jp

Kazuhiro Sato (Associate Professor, Curator) kazsato@rib.okayama-u.ac.jp

Daisuke Saisho (Assistant Professor, Genome analysis) saisho@rib.okayama-u.ac.jp

Makoto Ishii (Technician) makishii@rib.okayama-u.ac.jp


BARLEY GERMPLASM DATABASE  
on line search system for seed samples and ESTs


Publications
from Barley Germplasm Center

Catalogue of Baley Germplasm Preserved in Okayama University 1983
 
 

Special report of the Barley Germplasm Center (in Japanese with English Summary)

No.1 Studies on the breeding and evaluation of germplasm for the resistance to net blotch in barley

No.2 Studies on the breeding and evaluation for salt tolerance in barley (out of print)

No.3 Inheritance and gepgraphical distribtion of phenol reaction-less varieties of barley

No.4 Studies on the Fusarium head blight resistance in barley (out of print)

No.5 Studies on expressivity of hull-cracked grain in malting barley

No.6 Studies on the breeding and evaluation of germplasm for deep-seeding tolerance in barley
 

All above publications are available upon request. Please ask us by e-mail.


Sample request for seeds and DNA clones

Limited amount (20 kernels each for cultivated barley, 10 kernels each for wild barley and experimental line) of seed samples and DNA clones (cDNA and BAC) for research purpose are available upon request. Users must follow the MTAs below. Please contact the curator Kazuhiro Sato (kazsato@rib.okayama-u.ac.jp) to receive Order and Assurance form.


Barley Germplasm Center

Okayama University

MATERIAL TRANSFER AGREEMENT

(MTA)

 

This Material Transfer Agreement sets forth the terms and conditions under which Barley Germplasm Center, Okayama University (hereinafter referred to as the fBARLEY GERMPLASM CENTERf) will provide with the RECIPIENT, and the RECIPIENT will receive, the biological material specified as described in the assurance form and/or its derivatives (hereinafter referred to as the fMATERIALf) in response to the RECIPIENTfs request, and with which the RECIPIENT staff and organization agree before the RECIPIENT receives the MATERIAL:

 

The RECIPIENT shall inform the BARLEY GERMPLASM CENTER for itfs written consent on the usage of the MATERIAL for any other purposes than the purpose specified in the assurance form.

 

The RECIPIENT agrees to expressly describe the acknowledgement of the BARLEY GERMPLASM CENTER as the source of the MATERIAL in any publication reporting the use thereof and the RECIPIENT shall send a copy of such publication to the BARLEY GERMPLASM CENTER. The BARLEY GERMPLASM CENTER may disclose publicly, copy or otherwise use such publication to promote the contribution by the BARLEY GERMPLASM CENTER.

 

The RECIPIENT shall bear the cost of shipping, handling, part of production and other expenses necessary for preparation or distribution of the MATERIAL (except small number@of seed samples or DNA clones) for the RECIPIENT.

 

The BARLEY GERMPLASM CENTER agrees with the access to the MATERIAL only to those co-workers and students who work for the purpose specified in the assurance form under the direct supervision and responsibility of the RECIPIENT.

 

The RECIPIENT shall not distribute, resell or otherwise dispose of the MATERIAL and ITS DERIVATIVES to any third party. The disposition hereunder shall include any acts to transfer all or any part of the intellectual property or grant a license thereunder with respect to the MATERIAL.

 

Nothing in this AGREEMENT shall be interpreted that the BARLEY GERMPLASM CENTER grants the RECIPIENT any rights under any patents or other intellectual property, or licenses thereunder with respect to the MATERIAL.

 

The RECIPIENT assumes all liability for claims against the RECIPIENT and the BARLEY GERMPLASM CENTER by third parties relating to alleged infringement of any patent, copyright, trademark or other intellectual property rights, which may arise from the use, storage or disposal by the RECIPIENT of the MATERIAL, except for the case that the claim is caused by the gross negligence or willful misconduct of the BARLEY GERMPLASM CENTER.

 

The RECIPIENT acknowledges that the MATERIAL delivered pursuant to this AGREEMENT may have defective, hazardous or faulty properties and may not necessarily fit for a particular purpose and that the RECIPIENT assumes all liability for any consequences resulting from the use by the RECIPIENT of the MATERIAL.

 

The RECIPIENT agrees that any handling or other activities undertaken in their laboratory with the MATERIAL and ITS DERIVATIVES shall be conducted in compliance with all applicable laws, regulations and guidelines. The RECIPIENT shall, if necessary, take any steps or procedures to comply with legal requirements for handling of the MATERIAL.

 

Both parties shall discuss to enable amicable resolution of any accidents during shipment of the MATERIAL.

 

Where the RECIPIENT is in breach of this AGREEMENT, the BARLEY GERMPLASM CENTER may request the RECIPIENT to cease its subsequent use of the MATERIAL and other resources of the BARLEY GERMPLASM CENTER.

 

Both parties shall discuss in good faith to enable the amicable resolution of matters, arising in connection with the interpretation or performance hereof as well as the matters which are not expressly set forth in this AGREEMENT.

 

Any matter or dispute, which cannot be settled through said amicable discussion, shall be subject to the exclusive jurisdiction of Tokyo District Court, Japan. This AGREEMENT shall be governed in accordance with the laws of Japan.

 


Page Last modified: August 8, 2006 
Send comments to: kazsato@rib.okayama-u.ac.jp